Composite

Part:BBa_K3221014

Designed by: Jia Yan   Group: iGEM19_SCU-China   (2019-10-15)


pTPS1 - CNS3 - tCYC1

We construct this part to test whether pTPS1 is an appropriate promotor for CNS3 in yeast BY4742.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 599
    Illegal XbaI site found at 3702
    Illegal SpeI site found at 2363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 599
    Illegal NheI site found at 2391
    Illegal SpeI site found at 2363
    Illegal NotI site found at 605
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 599
    Illegal BglII site found at 2835
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 599
    Illegal XbaI site found at 3702
    Illegal SpeI site found at 2363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 599
    Illegal XbaI site found at 3702
    Illegal SpeI site found at 2363
    Illegal AgeI site found at 3765
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None